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M94A2716.TXT
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1994-10-25
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Document 2716
DOCN M94A2716
TI Detection of HIV-1 and HIV-2 antibodies using a newly developed HIV-1
and HIV-2 combined passive hemagglutination assay.
DT 9412
AU Jyomura S; Research Center, Dainabot Co., Ltd.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):240 (abstract no. PB0388). Unique
Identifier : AIDSLINE ICA10/94369859
AB OBJECTIVE: To evaluate the sensitivity and specificity of a newly
developed combined HIV-1/HIV-2 Hemagglutination assay. METHOD: A Passive
Hemagglutination Assay (PHA) for the simultaneous detection of
antibodies to HIV-1 and HIV-2 was developed using HIV-1 viral gp41,
recombinant HIV-1 p24, and recombinant HIV-2 p36 antigens. The assay
method utilizes serum or plasma of 1:16 dilution and triple antigen
coated duracytes. The specificity evaluation was performed by testing
populations of negative human plasma and HIV antibody negative
autoimmune panels. Sensitivity was evaluated by testing confirmed
Western blot positive HIV-1 and HIV-2 samples and commercially available
HIV-1 seroconversion panels (BBI). RESULTS: More than 99.9% of
specificity was obtained when 1,000 samples from healthy individuals and
79 samples from the patients of autoimmune diseases were tested. One
hundred percent of detectability was obtained by testing 30 of HIV-1 and
100 of HIV-2 confirmed blot positive samples. In eight seroconversion
panels (total 58 members), current Combo PHA detected 30/58 (52%) and
Western blot detected 26/58 (45%). DISCUSSION/CONCLUSION: Current HIV-1
and HIV-2 combined PHA could detect both anti HIV-1 and HIV-2 antibodies
with high sensitivity and specificity.
DE *AIDS Serodiagnosis Blotting, Western *Hemagglutination Tests Human
HIV Antibodies/*BLOOD HIV Antigens/IMMUNOLOGY HIV
Seropositivity/*DIAGNOSIS/IMMUNOLOGY HIV-1/*IMMUNOLOGY
HIV-2/*IMMUNOLOGY Predictive Value of Tests MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).